The role of the Research Officer/ Assistant will play a major role in focussing on the critical problem of improving the efficiency of iT cell production, by identifying and unlocking potential differentiation ” bottle necks”, inducing specific T cell lineages and increasing anti-cancer functionality. The outcomes will be of major significance for the Company.

Key Skills:

The successful applicant will have the appropriate knowledge base and “hands-on” skills in one or more of the following technologies:

1. In vitro culture of human immune cells (especially T cells) ideally with an understanding of their biology and function, including handling primary blood/ tissue samples, use of multi-parameter flow cytometry and in vitro cytotoxicity assays.
2. Experience in animal handling (mice) and analysing immune responses of cytotoxic cells to transplanted human cancer cells (i.v, i.p, subcutaneous).
3. Propagation of human-induced pluripotent stem cells (iPSCs) and experience in their directed differentiation.
4. General molecular biology methods including the production and use of CRISPR Cas9 gene editing and associated cell biology; PCR.
5. Detailed knowledge of the maintenance, quality controls and sterility requirements of clinic-ready tissue culture laboratories.
6. Organisational skills required for data management and documentation of standard operating procedures.

They will relish working in a challenging dynamic, interactive and very exciting environment.

The initial specialized technologies to be upskilled, under guidance, by the successful applicant will be:

1. Molecular biology and CRISPR based gene editing of iPSC
2. Culture, cloning and immune differentiation of gene-edited iPSCs into T cells (abTCR and gdTCR)
3. Flow cytometric analysis of differentiated cells
4. in vitro xCELLigence and in vivo bioluminescence cytotoxicity assays

These are critical core technologies performed at Cartherics.