The role of the Research Officer/ Assistant will play a major role in focussing on the critical problem of improving the efficiency of iT cell production, by identifying and unlocking potential differentiation ” bottle necks”, inducing specific T cell lineages and increasing anti-cancer functionality. The outcomes will be of major significance for the Company.
The successful applicant will have the appropriate knowledge base and “hands-on” skills in one or more of the following technologies:
- In vitro culture of human immune cells (especially T cells) ideally with an understanding of their biology and function, including handling primary blood/ tissue samples, use of multi-parameter flow cytometry and in vitro cytotoxicity assays.
- Experience in animal handling (mice) and analysing immune responses of cytotoxic cells to transplanted human cancer cells (i.v, i.p, subcutaneous).
- Propagation of human-induced pluripotent stem cells (iPSCs) and experience in their directed differentiation.
- General molecular biology methods including the production and use of CRISPR Cas9 gene editing and associated cell biology; PCR.
- Detailed knowledge of the maintenance, quality controls and sterility requirements of clinic-ready tissue culture laboratories.
- Organisational skills required for data management and documentation of standard operating procedures.
They will relish working in a challenging dynamic, interactive and very exciting environment.
The initial specialized technologies to be upskilled, under guidance, by the successful applicant will be:
- Molecular biology and CRISPR based gene editing of iPSC
- Culture, cloning and immune differentiation of gene-edited iPSCs into T cells (abTCR and gdTCR)
- Flow cytometric analysis of differentiated cells
- in vitro xCELLigence and in vivo bioluminescence cytotoxicity assays
These are critical core technologies performed at Cartherics.